Bt549三阴性乳腺癌
WebApr 18, 2024 · Triple-negative breast cancer cell line BT549 was cultured with 10%fetal bovine serum (Gibco, United States) in Roswell Park Memorial Institute (RPMI) 1640 with 1% p/s. Breast endothelial cell line MCF-10A was cultured in MCF-10A special medium (Procell, China). All the cells were cultured at 5% CO 2, 37℃. WebDec 4, 2024 · On the basis of the above results, endogenous PD-L1 in MDA-MB231 and BT549 cells was knocked out and substituted with PD-L1 Flag or PD-L1 C272A-Flag (termed as MB231 KO-PD-L1 Flag, MB231 KO-PD-L1 ...
Bt549三阴性乳腺癌
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WebWe used 5μM olaparib or 5μM RI-1, a Rad51 inhibitor, to treat PTEN-proficient BT549 cells respectively. The immunofluorescent analysis showed the combination of olaparib and RI-1 induced more than 4-fold of γH2AX foci than either of them. MTT assay showed 5μM RI-1 did not change the survival of PTEN-proficient BT549 cells, however, this ... Web(A) Breast cancer HS578T and BT549 cells in 96-well plates were treated with SRI37892 at the indicated concentrations for 96 h. Cell viability was measured by the CellTiter-Glo assay.
WebMay 5, 2024 · BT549 cells with GFP or MIG‐6 knockdown were seeded in a 6 cm cell culture plate for 24 h followed by starvation in serum‐free and glucose‐free DMEM for 16 h. Afterward, cells were incubated with the serum‐free and glucose‐free DMEM in the absence and presence of 50 µM 2‐NBDG for 15 and 30 min, respectively. Glucose uptake was … WebCCLE Name: BT549_BREAST. COSMIC ID: 905951. Sanger ID: 1835. Cell Line Passport: SIDM00122. Cellosaurus RRID: CVCL_1092. Growth Pattern: Adherent. Disease Invasive Breast Carcinoma Disease Subtype Breast Invasive Carcinoma, NOS Lineage. Breast. Lineage Subtype Invasive Breast Carcinoma. Lineage Sub-subtype ...
WebMay 17, 2024 · BT549 and HS578T cells were transfected with si_FST and si_Ctrl at a final concentration of 10 μM/well and interferin transfection reagent (Polyplus) according to the manufacturer’s recommendations when the cells reached 40–60% confluency. After the cells were incubated for 24 h at 37 °C after transfection, the culture media were replaced ... WebBT-549 (人乳腺管癌细胞) (STR鉴定正确) CL-0041 1×10⁶cells/T25培养瓶 10% FBS ＋0.01mg/ml Insulin＋ 1% P/S 数量： - + 加入购物车 说明书 在线咨询 推荐搭配 套餐一： …
WebMar 24, 2024 · 人TNBC细胞系BT549敲低SOX4 或ITGB6后细胞表面MHC-I类分子升高（可能由干扰素刺激引起）。 染色质免疫沉淀（CHIP）试验，可以看到SOX4特异峰多在干 …
WebBT-549. HTB-122 ™. BT-549 cells are epithelial cells isolated in 1978 from a papillary, invasive ductal tumor which had metastasized to 3 of 7 regional lymph nodes. The cells … penman family standWebJul 29, 2024 · 三阴性乳腺癌的病因尚不十分明确，年龄因素，内分泌、家族遗传等都被认为是三阴性乳腺癌的危险因素。 目前，化疗是三阴性乳腺癌最主要的全身治疗方法，手术 … tno hyperborea leaderWebApr 11, 2024 · 经过8年努力，一项由复旦大学附属肿瘤医院乳腺外科主任邵志敏教授领衔的三阴性乳腺癌临床试验研究成果，在全球肿瘤学顶尖杂志《Journal of Clinical Oncology》在线发表，影响因子28分。 penman fairs petersfieldWebJul 14, 2014 · BT549 cells were transfected with control or Fra-1 siRNA. Next day, the cells were transfected with either control vector or Fra-1 expression plasmid (OriGene) as indicated, and WST-1 assays of cell metabolic activity were carried out at the indicated time points. Western blot analysis confirms knockdown and rescue of Fra-1 levels. β-Actin ... penman clothing canadaWebApr 29, 2015 · Based on published protocols, we implanted 5 mio BT549 cells ortotopic in the mammary fad pad of 8 weeks old female NOD-SCID mice. The cells were injected with 50% Matrigel, in a total of 150 µl ... tno human factorstno importheffingWebJul 4, 2024 · 三阴性乳腺癌 (Triple-negative breast cancer;TNBC) 占乳腺癌的 10-15% ，2024年新发TNBC就有6~8万例，它是一种严重侵袭和高度增殖的亚型，在年轻女性群体中占比更高。 预后很差，大部分患者在三年后复发，在确诊前五年死亡。 化疗仍然是早期和晚期TNBC患者的主要治疗手段 ，这是一个高度未满足的医疗需求领域，急需改善治疗方 … tno ideology chart