Head kidney macrophages

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August undefined, 2024

WebThe salmonid head kidney is a hematopoietic organ and contains many T and B lymphocytes, macrophages, and melano-macrophages [50,51,52]. Interestingly, the SHK-1 cell line can synthesize melanin [ 53 ], and melanogenesis pathway genes have shown a high expression level in salmon head kidney and SHK-1 cells [ 51 , 53 ].

Head Kidney - an overview ScienceDirect Topics

WebJul 1, 2005 · Previous studies have demonstrated that beta-glucans stimulate Atlantic salmon, Salmo salar L., head kidney macrophages both in vitro and in vivo and increase protection against various pathogens. WebDuring Vibrio harveyi infection, the gut-liver axis was affected, since intestinal inflammation and dysbacteriosis were observed concomitantly with the appearance of hepatic nodules, besides the typical symptoms in the heart and kidney (Deng et al.). Head kidney macrophages of Atlantic salmon fed with a fish meal and fish oil diet, showed the ... motor vehicle gift transfer form 14-317

Butyrate-induced IL-22 expression in fish macrophages …

WebJan 24, 2024 · Thus, after kidney injury, the kidney-resident macrophages are a distinct cellular subpopulation that does not differentiate from nonresident, infiltrating, precursor immune cells. WebAcute kidney injury (AKI) is defined by a rapid decline in renal function. Regardless of the initial cause of injury, the influx of immune cells is a common theme during AKI. ... The … WebHead kidney macrophages from Atlantic cod, Gadus morhua L., were isolated by density sedimentation and maintained under serum-free conditions for up to one … motor vehicle green card

Identification and characterization of a novel NOD-like receptor …

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WebAug 30, 2024 · The head-kidney macrophages were isolated as described previously with slight modifications (Liu et al., 2014). Briefly, the head kidney was removed aseptically … WebM2 macrophages are usually termed in the literature as anti-inflammatory and wound healing. Substantial progress has been made in elucidating the biology of M2 … motor vehicle govWebThe effects of these recombinant proteins were assessed in vitro using head kidney macrophages of gilthead seabream (Sparus aurata L., Perciformes, Sparidae) and rainbow trout (Oncorhynchus mykiss W., Salmoniformes, Salmonidae). In both species, 3 h of stimulation with rFla and rND1 induced expression of the proinflammatory cytokines ... healthy food diets 2021

"WebBy day 29 head kidney macrophages were isolated and subjected to in vitro poly I:C stimulation to simulate viral infection, and samples were collected over 48 h. By day 58 fish were subjected to in vivo stimulation using poly I:C or a Vibrio water-based vaccine to simulate viral or bacterial infection, respectively. The fish were monitored for ... " - Head kidney macrophages

Head kidney macrophages

Isolation and characterization of head kidney derived macrophages …

WebThe LycCXCL8_L3 transcript was constitutively expressed in all examined tissues and significantly up-regulated in the spleen and head kidney tissues by inactivated trivalent bacterial vaccine. The LycCXCL8_L3 transcript was also detected in peripheral blood leukocytes (PBLs), primary head kidney macrophages (PKM), and large yellow croaker … WebApr 24, 2014 · The role of calcium (Ca 2+) and its dependent protease calpain in Aeromonas hydrophila-induced head kidney macrophage (HKM) apoptosis has been reported.Here, we report the pro-apoptotic involvement of calmodulin (CaM) and calmodulin kinase II gamma (CaMKIIg) in the process.We observed significant increase in CaM …

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WebMelanomacrophage centers (MMCs) are aggregates of highly pigmented phagocytes found primarily in the head kidney and spleen, and occasionally the liver of many vertebrates. Preliminary histological … Web1 day ago · The role of microRNAs (miRNAs) in Mycobacterium fortuitum pathogenesis is not well illustrated. Using zebrafish kidney macrophages (ZFKM) we observed that M. fortuitum triggers miR-155 expression, and the TLR-2/NF-κB axis plays a key role in initiating the process. We report that mir-155 activates the pro-inflammatory Stat1/TBX21 …

WebPrimary cell cultures of head-kidney macrophages (MØ) were used for phagocytic and bactericidal assays against Edwar … Specific components of both the innate and adaptive immune systems of channel catfish were evaluated after supplementation of culture media with arginine (ARG) and/or glutamine (GLN). WebDec 1, 2024 · In addition, the head kidney macrophages (HKMs) in turbots were isolated and incubated with VD3 in vitro, the results showed that VD3 significantly promoted the …

WebOct 10, 2024 · The morphology of head kidney macrophages from the Caspian Kutum (a) and the Persian sturgeon (b) after 24 h of culture.Formation of giant cells in the Caspian kutum (c) and the Persian sturgeon (d) after 5 days of culture.E. coli was phagocytized by macrophages from Caspian kutum (e), and Persian sturgeon (f).Aggregations around … WebIn mammals, HSCs originate from the bone marrow, while in fish, the primary hematopoietic organ is the anterior (or head) kidney. A heterogeneous population of adherent leukocytes, containing monocytes and macrophages, amongst other cells, can be isolated from the head kidney using Percoll density gradient centrifugation (21, 25, 26).

WebApr 13, 2024 · The analysis of ROS production and phagocytic activities in head kidney macrophages upon (r)OnTRAP5b. Head kidney macrophages were stimulated with 100 or 500 μg/mL (r)OnTRAP5b or (r)MBP, and oxidation of DCFH-DA was measured for 30 min using excitation and emission filters of 488 and 525 nm, respectively (A).

WebCadmium (Cd) with no known functional role in any life-form has myriad of harmful effects. The present study was designed to elucidate the mechanism of Cd-induced oxystress generation and its impact on antioxidant and apoptosis signaling pathways in head kidney macrophage (HKM) of Channa punctatus Bloch. healthy food diet plateWebIn the present study, we have thoroughly investigated butyrate-induced production and function of IL-22 in fish macrophages. Our results demonstrated that short-chain fatty acids (SCFAs), major microbiota-derived metabolites, promoted the expression of IL-22 in head kidney macrophages (HKMs) of turbot (Scophthalmus maximus L.). healthy food diet plan to lose weightWebJun 10, 2024 · Head kidney is a unique immune organ of fish and plays a vital role in preventing the invasion of pathogens and maintaining the health of the body in large … healthy food diets for teensWebApr 14, 2024 · Carbon nanotubes (CNTs) have gained much attention due to their superb properties, which make them promising options for the reinforcing composite materials with desirable mechanical properties. However, little is known about the linkage between lung exposure to nanomaterials and kidney disease. In this study, we compared the effects … healthy food diets to lose weight fastWebComparative effects of different concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on immune responses of head-kidney macrophages isolated from large yellow croaker were studied in vitro. After exposing to serum-free medium for 1 day, cultured cells were incubated in mediu … motor vehicle greeleyWebFeb 1, 2024 · In the current study, we isolated macrophages from turbot head kidney, which is the largest organ of hematopoiesis in teleost fish until adulthood, comparable to the bone marrow in mammals [20]. Our analysis by RT-PCR and Western blot analysis confirmed that SCFAs induced IL-22 expression in fish macrophages, contributing to … motor vehicle glassWebMar 1, 1997 · Head kidney macrophages from Atlantic cod, Gadus morhua L., were isolated by density sedimentation and maintained under serum-free conditions for up to … motor vehicle glass replacement