Smmc 7221

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August undefined, 2024

Web31 Dec 2010 · INTRODUCTION. Lysophosphatidic acid (LPA), also known as an important lipid mediator, is a potent regulator of proliferation and migration in many cell lines (Van Meeteren and Moolenaar, 2007; Pua et al., 2009; Liu et al., 2010; Wang et al., 2010).Extracellular LPA is thought to be produced primarily by the autotaxin … Web18 Jun 2014 · (A, B and C) Anoikis analysis is shown for (A) SMMC-7221 cells expressing either a control vector or two different clones expressing AEG-1; (B) MHCC-97H cells expressing either NC siRNA control or two different siRNAs against AEG-1; and (C) HCC-LM3 cells expressing NC or two different siRNAs against AEG-1. Cells were trypsinized and re …

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Web1 Jul 2024 · Furthermore, compound 1 was evaluated for its cytotoxic activity against five human tumor cell lines (HL-60, SMMC-7221, A549, MCF-7, and SW480) in vitro, and displayed selective cytotoxicity against SMMC-7721 with IC 50 value of 16.78 ± 0.49 μM. Graphical abstract. WebSMMC-7721 is a human hepatocellular carcinoma (HCC) cell line that was established from a liver tumor of a Chinese patient. HCC is the most common type of liver cancer, and the … streaming cw

Levels of HBx, VEGF, and CEACAM1 in HBV-related hepatocellular ...

WebAngiocidin expressed in SMMC-7221 and HepG2 cells. The angiocidin expression in two kinds of liver cancer cells are examined by RT-PCR and western blot. Fig.1a sho ws tha t angiocidin pr oteins ar e detected in SMMC-7221 and HepG2 cells. There seems to be not significant differences in angiocidin expression between SMMC-7221 and HepG2 cells ... Web1 Sep 2024 · Recombinant RIPs from Momordica charantia have been shown to produce different degrees of inhibition on various tumor cells, including bladder (5637), colorectal (LoVo), breast (MCF-7), cervical (HeLa), liver (SMMC-7221), gastric (BGC823 and MGC803) and skin (B16) cancer . However, little has been reported about the efficacy of … Web5 Feb 2024 · The apoptotic rates of HepG2 and SMMC-7221 cells treated with brevilin A were found to be markedly increased. The expression levels of MMP-2 and MMP-9 were … streaming cute bodyguard sub indo

Acquisition of anoikis resistance through CD147 upregulation: A …

MiRNA-15a-3p inhibits the metastasis of hepatocellular carcinoma …

WebAmong them, ciprofloxacin acyl hydrazone (Figure 1B), was found to exhibit extensive antitumor activities in liver cancer cells (SMMC-7221), leukemia cells (L1210) and hu- man promyelocytic leukemia cells7. Furthermore, compounds containing furan group have been a European Review for Medical and Pharmacological Sciences 2024; 22: 4720-4729 WebThe results showed that brevilin A reduced cell viability and invasion in HepG2 and SMMC-7221 cells. The apoptotic rates of HepG2 and SMMC-7221 cells treated with brevilin A … rowan sororityWeb27 Jun 2012 · SMMC-7721, a human hepatoma carcinoma (HCC) cell line, was cultured in Dulbecco’s modified Eagle medium (DMEM, Invitrogen Corp., Grand Island, New York) supplemented with 10% FBS at 37°C under a mixture of 95% air and 5% CO 2. ... (separate comparisons for PC-3 and SMMC-7221 mouse models). Similarly, one-way ANOVA was … rowan south jersey login

"Web10 Mar 2014 · (D) SMMC-7221 and HepG2 cells were transfected with GFP-LC3 plasmid for 24 h followed by exposure to BSA, oleic acid (OA, 500 μmol/L) or PA (500 μmol/L) for an additional 12 h. GFP-LC3 punctate... " - Smmc 7221

Smmc 7221

Recombinant Balsamin induces apoptosis in liver and

Web10 Jan 2024 · SMMC-7221 and another HCC cell line Huh-7, which showed relatively low AGO2 protein expression, were transiently transfected with pCMV3-AGO2 and control pCMV3 vector, respectively. Increased production of AGO2 in both SMMC-7221 and Huh-7 was detected by western blot analysis 48 hours post transfection (Figures 6(a) and 6(g)). … WebStructured Review. ATCC. Smmc 7221 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Average 86 stars, based on …

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WebINIS Repository Search provides online access to one of the world's largest collections on the peaceful uses of nuclear science and technology. The International Nuclear Information System is operated by the IAEA in collaboration with over 150 members. Web1 Jan 2024 · SMMC-7221 and HepG2 cells were transiently transfected with miR-542-3p mimics, miR-542-3p inhibitor, or miR-NC. MTT assay of SMMC-7221 (C) and HepG2 (D) cells at 24, 48, and 72 h of transfection. * p < 0.5. (E) The colony-forming capacity of HCC cells was detected by colony formation assay after incubation for 2 weeks. * p < 0.5 vs. miR-NC.

Web3 Jan 2016 · Compound 25 (Figure 9) with a propionyloxy group at the 4-position of the left phenyl ring showed the most potent inhibition activity which inhibited the growth of HepG2, A549, A375, SMMC-7221, and K562 cancer cell lines with IC 50 values of 0.15, 0.36, 0.62, 0.61, and 0.52 μM, respectively. Web29 Nov 2024 · Previously, our group performed in vitro work to investigate the role of miR-30a-5p on the biological function of HCC cell lines HepG2, SMMC-7221, HepB3, and SNU449. We transfected miR-30a-5p inhibitor and miR-30a-5p mimic into HCC cell lines and found that miR-30a-5p mimic could obviously inhibit cell growth and also induce caspase …

WebThe cytotoxicity induced by brucine from the seed of Strychnos nux-vomica proceeds via apoptosis and is mediated by cyclooxygenase 2 and caspase 3 in SMMC 7221 cells. To … Web21 Feb 2024 · SMMC-7221 cells were cotransfected with luciferase reporter vector, Renilla luciferase control vector (pRL-hTK) and miR-7 mimics or the negative control miRNA, …

Web27 Mar 2012 · The human SMMC-7221 human HCC cell line was used. Immunofluorescence was used to investigate the expression levels of CD147. Anoikis-induced cell death was assessed using trypan blue exclusion. In the present study, the results showed that SMMC-7721 HCC cells exhibited significant morphological changes …

Web14 Jan 2024 · SMMC-7721 cells transfected with STOML2-Flag were lysed in RIPA buffer and then loaded to Flag antibody and protein A/G agarose beads (Santa Cruz, sc-2003), the beads were washed with RIPA buffer for 5 times. Proteins complex was eluted using loading buffer, separated on SDS-PAGE gel and silver stained. rowan southlakeWeb18 Feb 2016 · SMMC-7221 cells were transferred into 24-well plate, and cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing antibiotics and 10 % (v/v) fetal … rowan southern baptist associationWeb16 May 2024 · LM3, SMMC-7221, and MHCC-97H cells were maintained in Roswell Park Memorial Institute 1640 (RPMI-1640, Gibco) medium with 10% foetal bovine serum (FBS, Sigma‒Aldrich) and 1% penicillin/streptomycin (Life Technology) at 37°C with 5% CO2. rowan sorbus treeWeb17 Oct 2024 · Interestingly, cellular morphology analysis showed that Huh7 and SMMC-7221 cells had an epithelial morphology in sh-NQO1 cells, while the morphology of NQO1 … streaming cxWebIn present study, we found that monocyte cell line THP-1 cells were more sensitive to Icaritin than tumor cell lines such as SMMC-7221 (Fig. 4C). We speculate that Icaritin may exert its effect mainly on host immune cells, particularly those with monocyte lineage, such as macrophages and myeloid DCs, which activate T cells by antigen presenting. streaming cw tvWeb13 Jul 2024 · Some researchers also extracted the active components from Bupleurum spp. callus (BSW-ws) and administered SMMC-7221 to cultured liver cancer cells at concentrations of 10, 20, 30, 40, 60, 80, 100 μg•mL-1 ; MTT method was used to detect the cytotoxic activity of BSW-ws, and the effect of BSW-ws on cell apoptosis was detected by … rowan song taylor swiftWebSMMC-7221, Huh7, Hep3B) and a normal hepato - cyte cell line (LO2) were purchased from Amer-ican Type Culture Collection (ATCC; Manassas, VA, USA). The cells were cultured in Dulbec-co’s Modified Eagle’s Medium (DMEM; Gibco, … streaming cycling today