T. thermophilus hb27
WebRecent work has shown that DNA can be transferred by conjugation from T. thermophilus strain HB8 to a closely related strain, HB27. Strain HB8 harbors a region of its chromosomal DNA similar in sequence to the F plasmid of E. coli which allows conjugal transfer of chromosomal genes after it integrates into the chromosome of the donor cell. WebT. thermophilus HB27 was originally isolated from a natural volcanic hot spring in Japan 43, and its complete genome sequence was reported as early as 2004 44. In 2016, Godde et al. 45 reported the presence of CRISPR repeat sequences and cas genes on the megaplasmid of T. thermophilus HB27, ...
T. thermophilus hb27
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WebApr 4, 2004 · The genome of T. thermophilus HB27 is composed of a 1,894,877 base pair (bp) chromosome (TTC) and a 232,605-bp megaplasmid (TTP), designated pTT27. … WebDec 4, 2014 · Organisms and cultivation. Escherichia coli was grown in lysogenic broth medium (LB) (Bertani 1951) containing 100 µg/µl ampicillin, 20 µg/µl kanamycin or 3 µg/µl bleomycin when appropriate. T. thermophilus HB27 was grown at 68 °C in TM + complex medium (Oshima and Imahori 1971).When appropriate, kanamycin (20 µg/µl in liquid, 40 …
WebThe ancestral thermophilic bacterium Thermus thermophilus strain HB27 encodes a Ppol protein among the genes present in mobile element ICETh2, absent in other T. thermophilus strains. WebLaboratory-adapted strains of Thermus spp. have been shown to require oxygen for growth, including the model strains T. thermophilus HB27 and HB8. In contrast, many isolates of …
WebThermus thermophilus HB27: 637000322: complete: JGI IMG/M. 262724 tax ID * Availability in culture collections External links [Ref.: #2956] Culture collection no. DSM 7039, ATCC … WebHenne A, Bruggemann H, Raasch C, Wiezer A, Hartsch T, Liesegang H, Johann A, Lienard T, Gohl O, Martinez-Arias R, et al. Title The genome sequence of the extreme thermophile Thermus thermophilus.
WebDec 7, 2024 · for T. thermophilus HB27, highly ef fi cient at temperatures up to 65°C. Using this tool, a T. thermophilus D TtarsM strain was constructed in less than 10 days (i ncluding the plasmid-
WebFeb 16, 2014 · For plasmid purification, T. thermophilus HB27 and HB27Δago cultures were cultivated in triplicates in TTH medium supplied with 30 ng μl −1 kanamycin and 100 ng μl −1 hygromycin. china palace restaurant newport beachWebThermus thermophilus HB27 is an extremely thermophilic eubacteria with a high frequency of natural competence. This organism is therefore often used as a thermophilic model to … grambling state football 2023 signeesWebWe cloned the genes encoding Mn-dependent catalases from Thermus thermophilus HB27 and HB8 and a less thermostable mutant carrying two amino acid replacements (M129V and E293G). When the wild-type and mutant genes were overexpressed in Escherichia coli, unmodified or six-His-tagged proteins of the expected size were overproduced as inactive … china palace steak houseWebComparison of multiple T. thermophilus genomes indicated that the genes for xylose metabolism were present on a plasmid in only one strain. Inspection of metagenomic dataset for adapted communities that contain T. thermophilus indicated that the plasmid is present in the T. thermophilus populations but may be lost upon isolation. KW - Consortium. grambling state football coachWebSep 16, 2010 · The HB8 strain can live in either anaerobe and aerobe; where as the HB27 can only strive in aerobe environment. HB8 survive anaerobeically in the presence of nitrate … china palace steak house menuWebIntense gene flux between prokaryotes result in high percentage of archaeal genes in the genome of the thermophilic bacteria Thermus spp. Among these archaeal genes a homolog to the Sulfolobus spp. HerA protein appears in all of the Thermus spp. strains so far sequenced (HepA). The role of HepA in Thermus thermophilus HB27 has been analyzed … china palace west chesterWebMay 5, 2024 · The wild-type DNA polymerase I gene from T. thermophilus HB27 (NCBI-protein ID: AAS81038) is 2505 nucleotides long with a GC content of 67.5% [14]. Although we first used this polymerase expressed in E. coli as a source of enzyme to amplify short and long target DNA sequences containing the polymerase gene itself, no amplification was … grambling state football 2022 schedule